rabbit anti mmp 13  (Bioss)

Journal: Frontiers in Pharmacology

Article Title: Combined multi-omics approach to identify the key metabolites, key microorganisms and biomarkers correlated with the neutrophil extracellular traps-associated gene TIMP1 in osteoarthritis

doi: 10.3389/fphar.2025.1665228

Figure Lengend Snippet: Validation of the TIMP1 gene silencing OA rat model. (A) Body weight changes in four groups during model construction. (B,C) Serum levels of TNF-α and IL-1β detected by ELISA. (D) Toluidine blue staining of cartilage tissue sections with semiquantitative analysis. (E) HE staining showing histological changes in cartilage tissue. (F) IHC staining and expression analysis of Collagen II, MMP3, MMP13, and TIMP1 in femoral cartilage tissues.

Article Snippet: For immunohistochemical analysis, 3 μm paraffin sections were incubated with primary antibodies against Collagen II (Huabio, ER1906-48, Hangzhou, China; 1:100), MMP3 (Affinity, AF0217, Liyang, China; 1:50), MMP13 (Bioss, bs-10581R, Beijing, China; 1:100), and TIMP-1 (Bioss, bs-4600R, Beijing, China; 1:100), all diluted in 2% BSA.

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry, Expressing

Journal: Frontiers in Pharmacology

Article Title: Combined multi-omics approach to identify the key metabolites, key microorganisms and biomarkers correlated with the neutrophil extracellular traps-associated gene TIMP1 in osteoarthritis

doi: 10.3389/fphar.2025.1665228

Figure Lengend Snippet: Validation of the TIMP1 gene silencing OA rat model. (A) Body weight changes in four groups during model construction. (B,C) Serum levels of TNF-α and IL-1β detected by ELISA. (D) Toluidine blue staining of cartilage tissue sections with semiquantitative analysis. (E) HE staining showing histological changes in cartilage tissue. (F) IHC staining and expression analysis of Collagen II, MMP3, MMP13, and TIMP1 in femoral cartilage tissues.

Article Snippet: For immunohistochemical analysis, 3 μm paraffin sections were incubated with primary antibodies against Collagen II (Huabio, ER1906-48, Hangzhou, China; 1:100), MMP3 (Affinity, AF0217, Liyang, China; 1:50), MMP13 (Bioss, bs-10581R, Beijing, China; 1:100), and TIMP-1 (Bioss, bs-4600R, Beijing, China; 1:100), all diluted in 2% BSA.

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry, Expressing

Journal: Scientific Reports

Article Title: Dihydrotestosterone and 17β-estradiol modulate TMJ osteoarthritis development and reveal sex-specific differences in pathogenesis

doi: 10.1038/s41598-025-03475-w

Figure Lengend Snippet: Effects of DHT reduction and mechanical stress on TMJ cartilage. (a) Histochemical staining of the mandibular head of a male mouse is shown. Cartilage thickness was confirmed by H-E staining (scale bar = 100 μm). Positive areas were measured by safranin-O staining (scale bar = 100 μm). The number of osteoclasts per mm 2 was measured by TRAP staining (scale bar = 100 μm).(b) Cartilage thickness measured on H-E stained sections. Proteoglycan area in Safranin-O stained sections. mean number of osteoclasts per mm 2 ( N > 3 nuclei) in TRAP stained sections. Results of the Modified Mankin score for OA severity assessment. (c) Immunohistochemical staining of the mouse mandibular head for MMP13 (scale bar = 100 μm; MMP13: red, nuclei: blue) and ADAMTS5 (scale bar = 100 μm; ADAMTS5: red, nuclei: blue). (d) Semi-quantitative analysis of MMP13 and ADAMTS5 expression comparing the control, MS, ORX, and MS + ORX groups. * P < 0.033, ** P < 0.002, *** P < 0.001, ns = no significance.

Article Snippet: Sections were incubated with the primary antibody (diluted in PBS supplemented with 1% BSA) according to procedures recommended by the manufacturer, as follows: ADAMTS5 rabbit polyclonal antibody as primary antibody (ab182795, Abcam, Cambridge, MA, USA) and MMP13 rabbit polyclonal antibody (LS-C352547, Proteintech, Chicago, IL, USA) as primary antibodies for immunohistochemical staining.

Techniques: Staining, Modification, Immunohistochemical staining, Expressing, Control

Journal: Scientific Reports

Article Title: Dihydrotestosterone and 17β-estradiol modulate TMJ osteoarthritis development and reveal sex-specific differences in pathogenesis

doi: 10.1038/s41598-025-03475-w

Figure Lengend Snippet: Impact of 17β-estradiol deficiency on TMJ degeneration under mechanical stress. (a) Histochemical staining of the mandibular head of a male mouse is shown. Cartilage thickness was confirmed by H-E staining (scale bar = 100 μm). Positive areas were measured by safranin-O staining (scale bar = 100 μm). The number of osteoclasts per mm 2 was measured by TRAP staining (scale bar = 100 μm).(b) Cartilage thickness measured on H-E stained sections. Proteoglycan area in Safranin-O stained sections. mean number of osteoclasts per mm 2 ( N > 3 nuclei) in TRAP stained sections. Results of the Modified Mankin score for OA severity assessment. ** P < 0.002, *** P < 0.001, ns = no significance. (c) Immunohistochemical staining of the mouse mandibular head for MMP13 (scale bar = 100 μm; MMP13: red, nuclei: blue) and ADAMTS5 (scale bar = 100 μm; ADAMTS5: red, nuclei: blue). ADMTS5 were clearly expressed in the subchondral bone in MS and MS + ORX group. (d) Semi-quantitative analysis of MMP13 and ADAMTS5 expression comparing the control, MS, Ai, and MS + Ai groups. * P < 0.033, ** P < 0.002, *** P < 0.001, ns = no significance.

Article Snippet: Sections were incubated with the primary antibody (diluted in PBS supplemented with 1% BSA) according to procedures recommended by the manufacturer, as follows: ADAMTS5 rabbit polyclonal antibody as primary antibody (ab182795, Abcam, Cambridge, MA, USA) and MMP13 rabbit polyclonal antibody (LS-C352547, Proteintech, Chicago, IL, USA) as primary antibodies for immunohistochemical staining.

Techniques: Staining, Modification, Immunohistochemical staining, Expressing, Control

Journal: Scientific Reports

Article Title: Dihydrotestosterone and 17β-estradiol modulate TMJ osteoarthritis development and reveal sex-specific differences in pathogenesis

doi: 10.1038/s41598-025-03475-w

Figure Lengend Snippet: Combined effects of DHT and 17β-estradiol deficiency with mechanical stress. (a) Histochemical staining of the mandibular head of a male mouse is shown. Cartilage thickness was confirmed by H-E staining (scale bar = 100 μm). Positive areas were measured by safranin-O staining (scale bar = 100 μm). The number of osteoclasts per mm 2 was measured by TRAP staining (scale bar = 100 μm).(b) Cartilage thickness measured on H-E stained sections. Proteoglycan area in Safranin-O stained sections. mean number of osteoclasts per mm2 ( N > 3 nuclei) in TRAP stained sections. Results of the Modified Mankin score for OA severity assessment. * P < 0.033, ** P < 0.002, *** P < 0.001, ns = no significance. (c) Immunohistochemical staining of the mouse mandibular head for MMP13 (scale bar = 100 μm; MMP13: red, nuclei: blue) and ADAMTS5 (scale bar = 100 μm; ADAMTS5: red, nuclei: blue). ADMTS5 were clearly expressed in the subchondral bone in MS, MS + Ai, MS + ORX and MS + ORX + Ai group. (d) Semi-quantitative analysis of MMP13 and ADAMTS5 expression comparing the control, MS, MS + Ai, MS + ORX, and MS + ORX + Ai groups. * P < 0.033, ** P < 0.002, *** P < 0.001, ns = no significance.

Article Snippet: Sections were incubated with the primary antibody (diluted in PBS supplemented with 1% BSA) according to procedures recommended by the manufacturer, as follows: ADAMTS5 rabbit polyclonal antibody as primary antibody (ab182795, Abcam, Cambridge, MA, USA) and MMP13 rabbit polyclonal antibody (LS-C352547, Proteintech, Chicago, IL, USA) as primary antibodies for immunohistochemical staining.

Techniques: Staining, Modification, Immunohistochemical staining, Tandem Mass Spectroscopy, Expressing, Control

Journal: Scientific Reports

Article Title: Dihydrotestosterone and 17β-estradiol modulate TMJ osteoarthritis development and reveal sex-specific differences in pathogenesis

doi: 10.1038/s41598-025-03475-w

Figure Lengend Snippet: Protective role of 17β-estradiol supplementation in TMJ-OA. Histochemical staining of the mandibular head of male mice is shown as control, E2− (MS + ORX + Ai) and E2+ (MS + ORX + Ai + E2). Cartilage thickness was confirmed by H-E staining (scale bar = 100 μm). Positive areas were measured by safranin-O staining (scale bar = 100 μm). The number of osteoclasts per mm 2 was measured by TRAP staining (scale bar = 100 μm).(b) Cartilage thickness measured in H-E stained sections, proteoglycan area in Safranin-O stained sections was thicker with E2 + than E2−; average number of osteoclasts per mm 2 ( N > 3 nuclei) in TRAP stained sections was reduced with E2 + Modified for OA severity assessment Mankin score for assessing OA severity was greater with E2+. ** P < 0.002, *** P < 0.001, ns = no significance. (c) Immunohistochemical staining of MMP13 (scale bar = 100 μm; MMP13: red, nuclei: blue) and ADAMTS5 (scale bar = 100 μm; ADAMTS5: red, nuclei: blue) in the mouse mandible head showed that expression was suppressed by E2+. (d) Semi-quantitative analysis of MMP13 and ADAMTS5 expression comparing the control, E2 + and E2 − groups. * P < 0.033, ** P < 0.002, *** P < 0.001, ns = no significance. (e) Quantitative evaluation of NFκβ , BMP7 , Gremlin1 , and Rela , mRNAs involved in TMJ-OA deterioration, by RT-qPCR. Bars indicate each mRNA level relative to GAPDH mRNA level ( n = 5) Data are shown as mean ± SD. * P < 0.033, ** P < 0.002, *** P < 0.001, ns = no significance.

Article Snippet: Sections were incubated with the primary antibody (diluted in PBS supplemented with 1% BSA) according to procedures recommended by the manufacturer, as follows: ADAMTS5 rabbit polyclonal antibody as primary antibody (ab182795, Abcam, Cambridge, MA, USA) and MMP13 rabbit polyclonal antibody (LS-C352547, Proteintech, Chicago, IL, USA) as primary antibodies for immunohistochemical staining.

Techniques: Staining, Control, Modification, Immunohistochemical staining, Expressing, Quantitative RT-PCR